Application of complex and chemically-defined medium supplements toward cell line specific performance enhancement of biopharmaceutical production systems
نویسندگان
چکیده
Introduction A series of cell-line specific complex media supplements have been developed for enhancing performance of various biopharmaceutical production systems. Created using in-house media optimization methods, these supplements are manufactured using innovative, proprietary process technology which allows for the combination of complex and/or chemically defined animal-component free media additives into a single homogeneous functional supplement. These supplements have been optimized for individual cell lines, and have proven to be suitable for use in a range of basal media. Data are presented which demonstrate the effectiveness of these optimized supplements as performance enhancers for application in CHO and SP2/0 batch culture, and as feed supplements in fed-batch systems. Preliminary data will also be presented on a parallel series of strictly chemically-defined supplements, similarly optimized for specific cell lines.
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